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Activation of satellite cells in the dorsal root ganglia in a disc-punctured rat model

Authors
  • Li, Yanjing1
  • Xi, Chunyang1
  • Niu, Ming2
  • Chi, Zhiyong1
  • Liu, Xiaoqi1
  • Yan, Jinglong1
  • 1 Harbin Medical University, Department of Orthopedic Surgery, The First Affiliated Hospital, 23 Youzheng St, Nangang District, Harbin, 150001, People’s Republic of China , Harbin (China)
  • 2 Harbin Medical University, Department of Mammary Surgery, The Third Affiliated Hospital, Harbin, 150001, People’s Republic of China , Harbin (China)
Type
Published Article
Journal
Journal of Orthopaedic Science
Publisher
Elsevier
Publication Date
May 26, 2011
Volume
16
Issue
4
Pages
433–438
Identifiers
DOI: 10.1007/s00776-011-0064-0
Source
Springer Nature
Keywords
License
Yellow

Abstract

BackgroundThe neural mechanisms underlying discogenic low back pain caused by disc degeneration remain unclear. Previous studies demonstrated that satellite cells (SC) play an important role in neuropathic pain.MethodsTwenty adult female Sprague-Dawley rats were used. The rats were divided into two groups: a nucleus pulposus (NP) group whose discs were punctured to expose the NP (n = 10) and a sham-operated group whose annulus fibrosus surface was scratched superficially (n = 10). In this study, we investigated the expression and cellular distribution of glial fibrillary acidic protein (GFAP, a marker of SC activation) in the dorsal root ganglia (DRG) innervating the intervertebral discs using a retrograde tracing method and immunohistochemistry in a disc-punctured rat model.ResultsIn the sham-operated group, GFAP-immunoreactive (IR) SCs were not detected. In the NP group, GFAP-IR SC became evident, and 49 ± 13% of neurons innervating the punctured discs were surrounded by GFAP-positive SCs.ConclusionsOur results were the first to provide evidence for a potential role of SCs in the neural mechanisms of discogenic low back pain caused by disc degeneration.

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