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Activation of a plant gene by T-DNA tagging: auxin-independent growth in vitro.

Authors
  • Hayashi, H
  • Czaja, I
  • Lubenow, H
  • Schell, J
  • Walden, R
Type
Published Article
Journal
Science (New York, N.Y.)
Publication Date
Nov 20, 1992
Volume
258
Issue
5086
Pages
1350–1353
Identifiers
PMID: 1455228
Source
Medline
License
Unknown

Abstract

A transferred DNA (T-DNA) tagging vector with the potential to produce dominant mutations was used with cocultured Agrobacterium tumefaciens and protoplasts to tag genes involved in the action of the plant growth substance auxin. Transgenic calli were selected for their ability to grow in the absence of auxin in the culture media. From one experiment, 12 calli that displayed this phenotype were recovered, of which 11 were able to regenerate into plants. In one plant studied in detail, protoplast division in the absence of auxin genetically cosegregated with a single T-DNA insert. A messenger RNA encoded by a 6.4-kilobase sequence of plant genomic DNA rescued from the mutant is overexpressed relative to untransformed plants. The genomic DNA, as well as a cognate complementary DNA, once transfected into protoplasts promote growth and cell division in vitro in the absence of exogenously added auxin.

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