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The activation of phosphatidylinositol-specific phospholipase C by insulin in mammary epithelial cells of lactating mouse.

Authors
  • Yoshimoto, A
  • Nakanishi, K
  • Anzai, T
  • Komine, S
Type
Published Article
Journal
Cell biochemistry and function
Publication Date
Jul 01, 1990
Volume
8
Issue
3
Pages
163–166
Identifiers
PMID: 2168815
Source
Medline
License
Unknown

Abstract

We have previously demonstrated in vitro that, in the endoplasmic reticulum and Golgi apparatus of mammary epithelial cells of lactating and pregnant mice, inositol 1,4,5-trisphosphate releases Ca2+ that has been stored in these organelles. In this study, we examined whether insulin and prolactin, essential for the growth of mammary gland and for lactation, influenced the activity of phosphatidylinositol-specific phospholipase C in mammary cells. In the plasma membrane fraction of mammary epithelial cells of the DDY mouse strain 5 days after the start of lactation after the first pregnancy, and with phosphatidylinositol as substrate, it was shown that the activity of phospholipase C was enhanced by about four times in the presence of insulin compared with the control. Such enhancement was not found in the membrane fraction treated with prolactin.

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