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Activation of Drosophila hemocyte motility by the ecdysone hormone.

Authors
  • Sampson, Christopher J1
  • Amin, Unum
  • Couso, Juan-Pablo
  • 1 JMS Building, University of Sussex, Brighton BN1 9QG, East Sussex, UK.
Type
Published Article
Journal
Biology open
Publication Date
Dec 15, 2013
Volume
2
Issue
12
Pages
1412–1420
Identifiers
DOI: 10.1242/bio.20136619
PMID: 24285708
Source
Medline
Keywords
License
Unknown

Abstract

Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise during metamorphosis. It is not known as to how hemocytes become activated from a sessile state in response to such infectious and developmental cues, although the hormone ecdysone has been suggested as the signal that shifts hemocyte behaviour from quiescent to migratory at metamorphosis. Here, we corroborate this hypothesis by showing the activation of hemocyte motility by ecdysone. We induce motile behaviour in larval hemocytes by culturing them with 20-hydroxyecdysone ex vivo. Moreover, we also determine that motile cell behaviour requires the ecdysone receptor complex and leads to asymmetrical redistribution of both actin and tubulin cytoskeleton.

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