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Actin filaments around endothelial fenestrae in rat hepatic sinusoidal endothelial cells

Authors
  • Nagai, Toshihiro1, 2
  • Yokomori, Hiroaki3
  • Yoshimura, Kazunori4
  • Fujimaki, Kayo4
  • Nomura, Masahiko4
  • Hibi, Toshifumi2
  • Oda, Masaya5
  • 1 Keio University, Electron Microscopy Laboratory, Tokyo, Japan , Tokyo
  • 2 Keio University, Department of Internal Medicine, Tokyo, Japan , Tokyo
  • 3 Kitasato Medical Center Hospital, Department of Internal Medicine, 121-1 Arai, Kitamotoshi, Saitama, 364-8501, Japan , Saitama
  • 4 Saitama Medical School, Department of Physiology, Saitama, Japan , Saitama
  • 5 International University of Health and Welfare, Organized Center of Clinical Medicine, Tokyo, Japan , Tokyo
Type
Published Article
Journal
Medical Electron Microscopy
Publisher
Springer-Verlag
Publication Date
Dec 01, 2004
Volume
37
Issue
4
Pages
252–255
Identifiers
DOI: 10.1007/s00795-004-0262-3
Source
Springer Nature
Keywords
License
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Abstract

The presence of microfilaments in the vicinity of sinusoidal endothelial fenestrae (SEF) suggests that the cytoskeleton of liver sinusoidal endothelial cells (LSEC) plays an important role in the modulation of SEF. In this study, we investigated actin filaments around SEF in LSECs. Monolayers of LSEC culture were established by infusing a rat liver with collagenase for 30 min and then culturing in RMPI medium for 24 h. Cells were reacted with 0.1% Triton X for 5 s and 15% glycerinated PHEM buffer (60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 2 mM MgCl, pH 6.9) containing heavy meromyosin for 10 min and observed under a transmission electron microscope. By electron microscopy with the modified heavy meromyosin decorated reaction, actin filaments were clearly demonstrated around SEF in LSEC.

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