The main objectives of the present paper were to verify antibacterial activity of Soleno stemma argel a medicinal plant used in folk medicine in Sudan for cure of many diseases. Five solvents with grade polarity were used to extract Solenostemma argel (petroleum ether, chloroform, ethyl acetate, ethanol and distilled water).These different plant extracts showed the presence of flavonoid, saponin and cardiac glycoside. Solenostemma argel leaves extracts were subjected to antibacterial assay using cup-plate agar diffusion method against one Gram positive bacterium Staphylococcus aureus and one Gram negative bacterium Escherichia coli. Four extracts except petroleum ether showed clear inhibitory action against both bacteria. The antibacterial activity is due to presence of phytoconstituents as saponin and flavonoid in different plant extracts which confirmed by Thin Layer Chromatography (TLC).