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ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Authors
  • García-Castro, Helena1
  • Kenny, Nathan J.1
  • Iglesias, Marta2, 3
  • Álvarez-Campos, Patricia1, 4
  • Mason, Vincent1
  • Elek, Anamaria2, 3
  • Schönauer, Anna1
  • Sleight, Victoria A.5
  • Neiro, Jakke6
  • Aboobaker, Aziz6
  • Permanyer, Jon2, 3
  • Irimia, Manuel2, 3, 7
  • Sebé-Pedrós, Arnau2, 3
  • Solana, Jordi1
  • 1 Oxford Brookes University, Oxford, UK , Oxford (United Kingdom)
  • 2 Barcelona Institute of Science and Technology (BIST), Barcelona, Spain , Barcelona (Spain)
  • 3 Universitat Pompeu Fabra (UPF), Barcelona, Spain , Barcelona (Spain)
  • 4 Universidad Autónoma de Madrid, Madrid, Spain , Madrid (Spain)
  • 5 University of Aberdeen, Aberdeen, UK , Aberdeen (United Kingdom)
  • 6 University of Oxford, Oxford, UK , Oxford (United Kingdom)
  • 7 ICREA, Barcelona, Spain , Barcelona (Spain)
Type
Published Article
Publication Date
Apr 08, 2021
Volume
22
Issue
1
Identifiers
DOI: 10.1186/s13059-021-02302-5
Source
Springer Nature
Keywords
License
Green

Abstract

Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life.

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