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Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity.

Authors
  • Choi, Chul Hee
  • Hyun, Sung Hee
  • Lee, Ji Young
  • Lee, Jun Sik
  • Lee, Yong Seok
  • Kim, Soon Ae
  • Chae, Jeong-Pil
  • Yoo, Seung Min
  • Lee, Je Chul
Type
Published Article
Journal
Cellular Microbiology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Feb 01, 2008
Volume
10
Issue
2
Pages
309–319
Identifiers
PMID: 17760880
Source
Medline
License
Unknown

Abstract

Acinetobacter baumannii is an emerging opportunistic pathogen responsible for healthcare-associated infections. The outer membrane protein A of A. baumannii (AbOmpA) is the most abundant surface protein that has been associated with the apoptosis of epithelial cells through mitochondrial targeting. The nuclear translocation of AbOmpA and the subsequent pathology on host cells were further investigated. AbOmpA directly binds to eukaryotic cells. AbOmpA translocates to the nucleus by a novel monopartite nuclear localization signal (NLS). The introduction of rAbOmpA into the cells or a transient expression of AbOmpA-EGFP causes the nuclear localization of these proteins, while the fusion proteins of AbOmpADeltaNLS-EGFP and AbOmpA with substitutions in residues lysine to alanine in the NLS sequences represent an exclusively cytoplasmic distribution. The nuclear translocation of AbOmpA induces cell death in vitro. Furthermore, the microinjection of rAbOmpA into the nucleus of Xenopus laevis embryos fails to develop normal embryogenesis, thus leading to embryonic death. We propose a novel pathogenic mechanism of A. baumannii regarding the nuclear targeting of the bacterial structural protein AbOmpA.

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