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Acetylcholinesterase biosensors based on ionic liquid functionalized carbon nanotubes and horseradish peroxidase for monocrotophos determination.

Authors
  • Jiaojiao, Xia1, 2
  • Bin, Zou3, 4
  • Pengyun, Wang5
  • Qing, Luo5
  • Xin, Shen5
  • Rui, Ji2
  • 1 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China. , (China)
  • 2 School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China. , (China)
  • 3 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China. [email protected] , (China)
  • 4 School of Food and Biological Engineering, Jiangsu University, No. 301 Xuefu Road, Zhenjiang, 212013, China. [email protected] , (China)
  • 5 School of Food and Biological Engineering, Jiangsu University, No. 301 Xuefu Road, Zhenjiang, 212013, China. , (China)
Type
Published Article
Journal
Bioprocess and Biosystems Engineering
Publisher
Springer-Verlag
Publication Date
Feb 01, 2020
Volume
43
Issue
2
Pages
293–301
Identifiers
DOI: 10.1007/s00449-019-02226-2
PMID: 31602490
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Long-term and excessive use of monocrotophos (MPs) pesticide leads to an accumulation of MPs residues in agricultural products. Electrochemical biosensor technology was developed as a simple and efficient method for detecting MPs. However, commercial acetylcholinesterase (AChE) sensors are not applied in practical MPs detection due to poor stability and reliability. In this study, the advantages of functionalized carbon nanotubes (Cl/MWCNTs) and a bi-enzyme system (horseradish peroxidase (HRP)/AChE) were combined, a novel bi-enzyme electrode (Cl/MWCNTs/HRP/AChE/GCE) was constructed. Under optimal conditions, the bi-enzyme sensor had a wide detection range of 1.0 × 10-11 to 1.0 × 10-7 mol/L and low detection limit of 4.5 × 10-12 mol/L. The proposed AChE biosensor exhibited excellent stability and sensitivity for MPs determination and presented a promising tool for monitoring food safety.

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