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Acetylcholinesterase biosensor for carbaryl detection based on interdigitated array microelectrodes

Authors
  • Gong, Zhili1
  • Guo, Yemin1
  • Sun, Xia1
  • Cao, Yaoyao1
  • Wang, Xiangyou1
  • 1 Shandong University of Technology, School of Agricultural and Food Engineering, No.12, Zhangzhou Road, Zibo, Shandong, 255049, People’s Republic of China , Zibo (China)
Type
Published Article
Journal
Bioprocess and Biosystems Engineering
Publisher
Springer-Verlag
Publication Date
Apr 27, 2014
Volume
37
Issue
10
Pages
1929–1934
Identifiers
DOI: 10.1007/s00449-014-1195-4
Source
Springer Nature
Keywords
License
Yellow

Abstract

In this study, an acetylcholinesterase (AChE) biosensor with superior accuracy and sensitivity was successfully developed based on interdigitated array microelectrodes (IAMs). IAMs have a series of parallel microband electrodes with alternating microbands connected together. Chitosan was used as the enzyme immobilization material, and AChE was used as the model enzyme for carbaryl detection to fabricate AChE biosensor. Electrochemical impedance spectroscopy was used in conjunction with the fabricated biosensor to detect pesticide residues. Based on the inhibition of pesticides on the AChE activity, using carbaryl as model compounds, the biosensor exhibited a wide range, low detection limit, and high stability. Moreover, the biosensor can also be used as a new promising tool for pesticide residue analysis.

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