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Acetylation as a means of selectively blocking vicinal diols of carbohydrates in electron microscopy.

Authors
  • Hirabayashi, Y
  • Yamada, K
Type
Published Article
Journal
Acta Histochemica
Publisher
Elsevier
Publication Date
Jan 01, 1986
Volume
80
Issue
2
Pages
235–238
Identifiers
PMID: 2433885
Source
Medline
License
Unknown

Abstract

Acetylation for selectively blocking the vicinal diols of carbohydrates has successfully been applied to ultrathin sections in electron microscopy. From buffered glutaraldehyde fixed rat colon and jejunum tissues embedded in LR-White, ultrathin sections were cut, treated with hot acetic anhydride in pyridine and then reacted for periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP). The PA-TCH-SP reaction of all the glycoproteins and glycogen involved in the colonic and jejunal epithelia was selectively blocked by prior acetylation, providing a means of substantiating the presence of vicinal diols of carbohydrates in electron microscopy.

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