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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors
  • Dignam, J D
  • Lebovitz, R M
  • Roeder, R G
Type
Published Article
Journal
Nucleic acids research
Publication Date
Mar 11, 1983
Volume
11
Issue
5
Pages
1475–1489
Identifiers
PMID: 6828386
Source
Medline
License
Unknown

Abstract

We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).

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