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Evaluation of intracellular lipids by standardized staining with a Sudan black B fraction

Authors
Journal
Journal of Biochemical and Biophysical Methods
0165-022X
Publisher
Elsevier
Publication Date
Volume
21
Issue
1
Identifiers
DOI: 10.1016/0165-022x(90)90040-j
Keywords
  • Cytophotometry
  • Neutral Lipid
  • Sudan Black B

Abstract

Abstract The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography. The cytophotometric assessment of intracellular neutral lipids, stained with SBB-I, is described and applied to the study of changes in granulocytic neutral lipids in leukemia.

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