The results showed that 3 TCM of Cinnamonum cassia, Eugenia caryophyllata and Pogostemon cablin have higher activity, and the parallel tests showed that the petroleum ether extract had higher activities (0.0046 mg/cm2, 0.005 mg/cm2 and 0.006 mg/cm2 respectively, 24 h, LD50) than the extracts of ethyl acetate and methanol. The acaricidal activity of the ethyl acetate extracts from C. cassia, P. cablin and Asarum sieboldii (0.00144 mg/cm2, 0.00347 mg/cm2 and 0.05521 mg/cm2 respectively, 24 h, LD50) were almost comparable to that of benzyl benzoate and dibutyl phthalate. However, the methanolic extracts of were less effective. CONCLUSIONS. This study shows the use of extracts with petroleum ether of C. cassia, P. cablin and E. caryophyllata as eco-friendly biodegradable agents for the control of the house dust mite.