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Hybridisation of short DNA molecules investigated with in situ atomic force microscopy

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Publisher
Elsevier BV
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Keywords
  • By Introducing The Complementary Dna (Cdna) Strand To A Molecular Layer Of Short Single Stranded Dna
  • Immobilised On A Gold Surface
  • We Have Investigated Hybridisation Between The Two Dna Strands Through The Technique Of In Situ Atom
  • Before Introduction Of Cdna
  • The Ssdna Molecular Layer Was Modulated With The Spacer Molecule Mercaptohexanol (Mch)
  • Which Makes The Ssdna Molecules More Accessible For Hybridisation
  • <Br/><Br/>With In Situ Afm
  • We Have Monitored The Formation Of A Smooth
  • Mixed Molecular Layer Containing Ssdna And Mch
  • Furthermore
  • The Hybridisation Between The Two Dna Strands Has Been Studied
  • Introduction Of The Cdna Strand Resulted In An Increase In Smoothness And Thickness Of The Molecular
  • Both The Increase In Order And Thickness Of The Molecular Layer Can Be Expected If Hybridisation Occ
  • Since Double Stranded Dna Molecules Have A More Rigid And Elongated Structure Than Ssdna Molecules
  • (C) 2003 Elsevier Science B
  • V
  • All Rights Reserved

Abstract

Hybridisation of short DNA molecules investigated with in situ atomic force microscopy - DTU Orbit (26/05/14) Hybridisation of short DNA molecules investigated with in situ atomic force microscopy - DTU Orbit (26/05/14) Holmberg M, Kuhle A, Garnaes J, Boisen A. Hybridisation of short DNA molecules investigated with in situ atomic force microscopy. Ultramicroscopy. 2003;97(1-4):257-261. Available from: 10.1016/S0304-3991(03)00050-0

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