Affordable Access

Abundance and diversity of Archaea in heavy-metal-contaminated soils.

Authors
  • Sandaa, R A
  • Enger, O
  • Torsvik, V
Type
Published Article
Journal
Applied and environmental microbiology
Publication Date
Aug 01, 1999
Volume
65
Issue
8
Pages
3293–3297
Identifiers
PMID: 10427009
Source
Medline
License
Unknown

Abstract

The impact of heavy-metal contamination on archaean communities was studied in soils amended with sewage sludge contaminated with heavy metals to varying extents. Fluorescent in situ hybridization showed a decrease in the percentage of Archaea from 1.3% +/- 0.3% of 4', 6-diamidino-2-phenylindole-stained cells in untreated soil to below the detection limit in soils amended with heavy metals. A comparison of the archaean communities of the different plots by denaturing gradient gel electrophoresis revealed differences in the structure of the archaean communities in soils with increasing heavy-metal contamination. Analysis of cloned 16S ribosomal DNA showed close similarities to a unique and globally distributed lineage of the kingdom Crenarchaeota that is phylogenetically distinct from currently characterized crenarchaeotal species.

Report this publication

Statistics

Seen <100 times