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Absence of TI-VAMP/Vamp7 leads to increased anxiety in mice.

Authors
  • Danglot, Lydia
  • Zylbersztejn, Kathleen
  • Petkovic, Maja
  • Gauberti, Maxime
  • Meziane, Hamid
  • Combe, Roy
  • Champy, Marie-France
  • Birling, Marie-Christine
  • Pavlovic, Guillaume
  • Bizot, Jean-Charles
  • Trovero, Fabrice
  • Della Ragione, Floriana
  • Proux-Gillardeaux, Véronique
  • Sorg, Tania
  • Vivien, Denis
  • D'Esposito, Maurizio
  • Galli, Thierry
Publication Date
Feb 08, 2012
Source
Hal-Diderot
Keywords
Language
English
License
Unknown
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Abstract

Vesicular (v)- and target (t)-SNARE proteins assemble in SNARE complex to mediate membrane fusion. Tetanus neurotoxin-insensitive vesicular-associated membrane protein (TI-VAMP/VAMP7), a vesicular SNARE expressed in several cell types including neurons, was previously shown to play a major role in exocytosis involved in neurite growth in cultured neurons. Here we generated a complete constitutive knock-out by deleting the exon 3 of Vamp7. Loss of TI-VAMP expression did not lead to any striking developmental or neurological defect. Knock-out mice displayed decreased brain weight and increased third ventricle volume. Axon growth appeared normal in cultured knock-out neurons. Behavioral characterization unraveled that TI-VAMP knock-out was associated with increased anxiety. Our results thus suggest compensatory mechanisms allowing the TI-VAMP knock-out mice to fulfill major developmental processes. The phenotypic traits unraveled here further indicate an unexpected role of TI-VAMP-mediated vesicular traffic in anxiety and suggest a role for TI-VAMP in higher brain functions.

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