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Absence of Dipeptidyl Peptidase 3 Increases Oxidative Stress and Causes Bone Loss.

Authors
  • Menale, Ciro1, 2
  • Robinson, Lisa J3
  • Palagano, Eleonora1, 2
  • Rigoni, Rosita1, 2
  • Erreni, Marco4
  • Almarza, Alejandro J5
  • Strina, Dario1, 2
  • Mantero, Stefano1, 2
  • Lizier, Michela1, 2
  • Forlino, Antonella6
  • Besio, Roberta6
  • Monari, Marta7
  • Vezzoni, Paolo1, 2
  • Cassani, Barbara1, 2
  • Blair, Harry C8
  • Villa, Anna1, 2
  • Sobacchi, Cristina1, 2
  • 1 Consiglio Nazionale delle Ricerche-Istituto di Ricerca Genetica e Biomedica (CNR-IRGB), Milan Unit, Milan, Italy. , (Italy)
  • 2 Humanitas Clinical and Research Center-Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rozzano, Italy. , (Italy)
  • 3 Department of Pathology, West Virginia University, Morgantown, WV, USA.
  • 4 Unit of Advanced Optical Microscopy, Humanitas Clinical and Research Center-Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rozzano, Italy. , (Italy)
  • 5 Department of Oral Biology, Department of Bioengineering, McGowan Institute of Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • 6 Department of Molecular Medicine, Unit of Biochemistry, University of Pavia, Pavia, Italy. , (Italy)
  • 7 Clinical Investigation Laboratory, Humanitas Clinical and Research Center-Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rozzano, Italy. , (Italy)
  • 8 Veterans' Affairs Medical Center and Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA.
Type
Published Article
Journal
Journal of Bone and Mineral Research
Publisher
Wiley (John Wiley & Sons)
Publication Date
Nov 01, 2019
Volume
34
Issue
11
Pages
2133–2148
Identifiers
DOI: 10.1002/jbmr.3829
PMID: 31295380
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Controlling oxidative stress through the activation of antioxidant pathways is crucial in bone homeostasis, and impairments of the cellular defense systems involved contribute to the pathogenesis of common skeletal diseases. In this work we focused on the dipeptidyl peptidase 3 (DPP3), a poorly investigated ubiquitous zinc-dependent exopeptidase activating the Keap1-Nrf2 antioxidant pathway. We showed Dpp3 expression in bone and, to understand its role in this compartment, we generated a Dpp3 knockout (KO) mouse model and specifically investigated the skeletal phenotype. Adult Dpp3 KO mice showed a mild growth defect, a significant increase in bone marrow cellularity, and bone loss mainly caused by increased osteoclast activity. Overall, in the mouse model, lack of DPP3 resulted in sustained oxidative stress and in alterations of bone microenvironment favoring the osteoclast compared to the osteoblast lineage. Accordingly, in vitro studies revealed that Dpp3 KO osteoclasts had an inherent increased resorptive activity and ROS production, which on the other hand made them prone to apoptosis. Moreover, absence of DPP3 augmented bone loss after estrogen withdrawal in female mice, further supporting its relevance in the framework of bone pathophysiology. Overall, we show a nonredundant role for DPP3 in the maintenance of bone homeostasis and propose that DPP3 might represent a possible new osteoimmunological player and a marker of human bone loss pathology. © 2019 American Society for Bone and Mineral Research. © 2019 American Society for Bone and Mineral Research.

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