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Abortion in a Mediterranean miniature donkey (Equus asinus) associated with a gammaherpesvirus similar to Equid herpesvirus 7.

Authors
  • LeCuyer, Tessa E1
  • Rink, Anette2
  • Bradway, Daniel S2
  • Evermann, James F2
  • Nicola, Anthony V2
  • Baszler, Timothy2
  • Haldorson, Gary J2
  • 1 Washington Animal Disease Diagnostic Laboratory (LeCuyer, Bradway, Evermann, Baszler, Haldorson), College of Veterinary Medicine, Washington State University, Pullman, WADepartment of Veterinary Microbiology and Pathology (LeCuyer, Nicola, Baszler, Haldorson), College of Veterinary Medicine, Washington State University, Pullman, WAPaul G. Allen School for Global Animal Health (Bradway, Nicola, Baszler), College of Veterinary Medicine, Washington State University, Pullman, WADepartment of Veterinary Clinical Sciences (Evermann), College of Veterinary Medicine, Washington State University, Pullman, WAAnimal Disease and Food Safety Laboratory, Nevada Department of Agriculture, Sparks, NV (Rink) [email protected]
  • 2 Washington Animal Disease Diagnostic Laboratory (LeCuyer, Bradway, Evermann, Baszler, Haldorson), College of Veterinary Medicine, Washington State University, Pullman, WADepartment of Veterinary Microbiology and Pathology (LeCuyer, Nicola, Baszler, Haldorson), College of Veterinary Medicine, Washington State University, Pullman, WAPaul G. Allen School for Global Animal Health (Bradway, Nicola, Baszler), College of Veterinary Medicine, Washington State University, Pullman, WADepartment of Veterinary Clinical Sciences (Evermann), College of Veterinary Medicine, Washington State University, Pullman, WAAnimal Disease and Food Safety Laboratory, Nevada Department of Agriculture, Sparks, NV (Rink).
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
November 2015
Volume
27
Issue
6
Pages
749–753
Identifiers
DOI: 10.1177/1040638715611444
PMID: 26462760
Source
Medline
Keywords
License
Unknown

Abstract

Fetal tissues and placenta from a third trimester Mediterranean miniature donkey (Equus asinus) abortion were submitted to the Washington State University, Washington Animal Disease Diagnostic Laboratory for abortion diagnosis. Microscopic examination of formalin-fixed tissues revealed multifocal necrotizing placentitis. Several cells within the necrotic foci contained large, eosinophilic, intranuclear inclusions. Virus isolation from fresh, frozen placenta identified a cytopathic, syncytia-forming virus. Polymerase chain reaction (PCR) from the cultured virus using degenerate universal herpesvirus primers amplified a 699-base pair portion of the DNA polymerase gene. The PCR amplicon had 96.7% nucleotide identity with the DNA polymerase gene of Equid herpesvirus 7 (EHV-7; asinine herpesvirus 2), a gammaherpesvirus. An identical sequence was obtained when the same degenerate herpesvirus primers were used for PCR on the formalin-fixed placenta. Additionally, the amplicon had complete identity with short sequences of asinine herpesviruses that have been published in association with interstitial pneumonia in donkeys. EHV-7 has previously been isolated from nasal secretions of normal donkeys and mules. Our report describes a case of abortion associated with EHV-7 or a similar virus.

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