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ABC protein CgABCF2 is required for asexual and sexual development, appressorial formation and plant infection in Colletotrichum gloeosporioides.

Authors
  • Zhou, Zongshan1
  • Wu, Jianyuan1
  • Wang, Meiyu1
  • Zhang, Junxiang2
  • 1 Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, Liaoning, China. , (China)
  • 2 Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, Liaoning, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Microbial Pathogenesis
Publisher
Elsevier
Publication Date
Sep 01, 2017
Volume
110
Pages
85–92
Identifiers
DOI: 10.1016/j.micpath.2017.06.028
PMID: 28645773
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

ATP-binding cassette (ABC) proteins are exclusively found in both prokaryotes and eukaryotes. In this study, we have characterized a gene from Glomerella leaf spot pathogen Colletotrichum gloeosporioides that encodes an ABC protein, whose function to date remains unknown. We designated this gene as CgABCF2. Deletion of CgABCF2 showed drastic reduction both growing rate and conidial production in C. gloeosporioides. The Δcgabcf2 mutant did not form the appressoria, lost the capability to infect apple and failed to form lesions on the wounded leaves and fruits. The C. gloeosporioides native CgABCF2 fully recovered defect of the Δcgabcf2 mutant. These data indicated that CgABCF2 was required for fungal development and invasion. The transcriptions of six pectolytic enzymes genes (CgPG1, CgPG2, pnl-1, pnl-2, pelA and pelB) significantly reduced in the Δcgabcf2 mutant, indicating that deletion of CgABCF2 impaired the fungal necrotrophic growth. In addition, CgABCF2 mediated sexual development through the positive regulation of the gene MAT1-2-1 expression. These results indicated that CgABCF2 underlies the complex process governing morphogenesis, sexual and asexual reproduction, appressorial formation and pathogenicity in C. gloeosporioides. Copyright © 2017 Elsevier Ltd. All rights reserved.

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