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New plastic plates which enhance neurite extension in culture: roles of bisphenol-A and tricyclodecanyl units for growth and orientation of neurites on plastic plates with microstructures

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
127
Issue
1
Identifiers
DOI: 10.1016/0304-3940(91)90883-u
Keywords
  • Neurite Growth
  • Neurite Orientation
  • Dorsal Root Ganglion Cell
  • Neuronal Cell Culture
  • Microstructure
  • Plastic
  • Hydrophilicity

Abstract

Abstract In order to study molecular mechanisms of contact guidance of neurites, dissociated culture of adult mouse dorsal root ganglion (DRG) cells was performed for 6 species of plastic plates different in monomer compositions. Microstructures (grooves of 5 10 μm wide and 1 μm deep) were grafted on the plates so as to guide neurite growth in their axial direction. Neurites were longer and more oriented on plates with larger water contact angle. The distribution of neurites was uneven between grooves and steps of the microstructures, exhibiting a bell-shaped curve in relation to the water contact angle of the plastic. These indicate that hydrophilic properties of the plastic plate surface, due to the presence of bisphenol-A and tricyclodecanyl units, are crucially involved in the elongation and orientation of neurites.

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