Affordable Access

Publisher Website

Growth of human oligodendrocytes in vitro on extracellular matrix

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
69
Issue
1
Identifiers
DOI: 10.1016/0304-3940(86)90408-8
Keywords
  • Extracellular Matrix (Ecm) Oligodendrocyte
  • Differentiation Of Human Cells
  • Galactocerebroside
  • Autoradiography
Disciplines
  • Biology

Abstract

Abstract Based on the successful use of a basement membrane-like extracellular matrix (ECM) as a physiological substrate for the growth of adult rat oligodendrocytes, we have applied the system to oligodendroglia of human origin. ECM is excreted and deposited on a culture dish by endothelial cells. Glial cells isolated from fresh human brains by a Percoll density gradient were maintained on ECM for several weeks in vitro. Cells identified as oligodendrocytes by anti-galactocerebroside (GalC) could be transferred and replated on ECM-coated plates. In one case, GalC-positive cells isolated from a biopsy of an 8-year old girl were able to incorporate [ 3H]thymidine as revealed by autoradiography. ECM can be used successfully for the growth and in vitro maintenance of human oligodendrocytes.

There are no comments yet on this publication. Be the first to share your thoughts.