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Mapping and initiation studies on the leader RNA of vesicular stomatitis virus

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
77
Issue
1
Identifiers
DOI: 10.1016/0042-6822(77)90423-8
Disciplines
  • Biology

Abstract

Abstract Vesicular stomatitis virus synthesizes a leader RNA in addition to the five monocistronic mRNA species in vitro. The leader RNA molecule appears to be an initiated RNA molecule since it is polyphosphorylated at its 5′-terminus. In addition, uv inactivation studies on purified virions suggest that leader RNA is synthesized prior to mRNA synthesis. The leader RNA is coded for at the 3′-end of genome RNA since purified leader RNA will protect the 3′-terminal region of the genome RNA from RNase digestion. Kinetic studies show that leader RNA and mRNA coding for the N protein anneal to 42 S genome RNA at the same rate, indicating that the leader RNA is coded for only once on the 42 S genome RNA. In the absence of UTP, an oligonucleotide is synthesized in vitro which contains the same 5′-terminal sequence as the leader RNA. These results indicate that the synthesis of the leader RNA is the first event during in vitro RNA transcription by vesicular stomatitis virus.

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