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A simple method for estimating oral microbial levels

Authors
Journal
Journal of Microbiological Methods
0167-7012
Publisher
Elsevier
Publication Date
Volume
9
Issue
4
Identifiers
DOI: 10.1016/0167-7012(89)90067-5
Keywords
  • Method
  • Methylene Blue
  • Milk
  • O2
  • Oral Microorganism

Abstract

Abstract A simple test is described which enables estimation of oral microbial levels. The test is based on three steps: (1) oral rinsing with ultrahigh-temperature-sterilized milk, (2) expectoration of the sample into a vessel containing methylene blue, and (3) measurement of the time required for colour change at the bottom of the vessel, indicating anaerobiosis. The results correlate highly with microbial counts (colony-forming units, aerobic conditions) in the expectorate. The method may have potential clinical applications as a test for measurement of oral hygiene levels.

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