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Identification of4-N-2- acetamido-2- deoxy-β- d - glucopyranosyl- l - asparaginein biological materials by gas chromatography-mass spectrometry

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
91
Issue
1
Identifiers
DOI: 10.1016/0009-8981(79)90473-x
Disciplines
  • Biology
  • Medicine

Abstract

Abstract A specific and sensitive method for the identification of 4-N-2- acetamido-2- deoxy-β- d - glucopyranosyl- l - asparagine (GlcNAc-Asn) in urine in asparatyl-glycosaminuria and in hydrolysates of glycoproteins is described. The method involves permethylation of GlcNAc-Asn followed by gas chromatographic-mass spectrometric analysis of the methylated derivative. It can be used to confirm the diagnosis of aspartylglycosaminuria and to assess the excretion of GlcNAc-Asn in urine during various phases of the disease. The presence of an N-acetylglucosaminyl-asparagine type of carbohydrate-peptide linkage in a glycoprotein can be determined by applying the method to the partial acid hydrolysate of a proteolytically digested glycoprotein.

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