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Analysis of Cooperativity by Isothermal Titration Calorimetry

Authors
Journal
International Journal of Molecular Sciences
1422-0067
Publisher
MDPI AG
Publication Date
Volume
10
Issue
8
Identifiers
DOI: 10.3390/ijms10083457
Keywords
  • Review
Disciplines
  • Biology
  • Physics

Abstract

Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems.

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