Electronic cell counting to measure total cell numbers in bronchoalveolar lavage fluid

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Electronic cell counting to measure total cell numbers in bronchoalveolar lavage fluid

  • In This Study
  • However
  • Cell Counting Of Bronchoalveolar Lavage (Bal) Fluid Is Performed Manually In Routine Practice
  • This Has Both Methodological And Inherent Errors
  • The Accuracy And Suitability Of Automated Counting Devices Have Been Questioned
  • A Coulter(R) Counter D Industrial Model Was Calibrated And Then Used To Measure The Total Cell Count
  • And Compared To A Standard Manual Method


Eur Respir J, 1994, 7, 1527–1531 DOI: 10.1183/09031936.94.07081527 Printed in UK - all rights reserved Copyright ERS Journals Ltd 1994 European Respiratory Journal ISSN 0903 - 1936 EElleeccttrroonniicc cceellll ccoouunnttiinngg ttoo mmeeaassuurree ttoottaall cceellll nnuummbbeerrss iinn bbrroonncchhooaallvveeoollaarr llaavvaaggee fflluuiidd L.G. Heaney*†, J. McKirgan**, C.F. Stanford*, M. Ennis†, Electronic cell counting to measure total cell numbers in bronchoalveolar lavage fluid. L.G. Heaney, J. McKirgan, C.F. Stanford, M. Ennis. ERS Journals Ltd 1994. ABSTRACT: Cell counting of bronchoalveolar lavage (BAL) fluid is performed manually in routine practice. This has both methodological and inherent errors; however, the accuracy and suitability of automated counting devices have been questioned. In this study, a Coulter® Counter D Industrial model was calibrated and then used to measure the total cell count in unprocessed bronchoalveolar lavage fluid, and compared to a standard manual method. Bronchoalveolar lavage was performed on 34 patients undergoing routine bron- choscopy. An aliquot of unprocessed bronchoalveolar lavage fluid was taken for all counting procedures. Manual counts were performed blind by two experienced independent observers using improved Neubauer chambers. Electronic counting measured 1 ml aliquots suspended in 10 and 20 ml Isoton® counting 0.5 and 1 ml duplicates. The correlation coefficients between electronic and manual counts were good. The coefficients of repeatability of electronic counts, for repeat counts, both on the same dilution (Intra-Coulter®: 0.1×105 cells·ml-1) and different dilutions (Interdilution- Coulter®: 0.48×105 cells·ml-1), were superior compared to those for repeat manual counts by the same observer (1.03×105 cells·ml-1), and counts between observers (1.82×105 cells·ml-1). This method offers a quick, precise and simple method for counting cells in unprocessed bronchoalveolar lavage fluid, which is both less labour intensiv

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