TMO electrodes provide a suitable platform for spectroscopy- and microscopy-based investigation of viable EABs. Commercially available TMOs, such as ITO, are produced from rare and expensive elements. Thus, there is a significant need to explore alternative technology to meet the demand. ZnO-based TMOs are low- cost and have excellent electrochemical properties. In this study, we report for the first time a model EAB of Shewanella loihica PV-4 on ZnO films deposited on perspex substrates via PLD. Commercially available ITO electrodes were also used for comparison. EAB electroactivity was characterized through MBV. ITO and ZnO films show flat surfaces and amorphous structure, and have 60 and 105 Ω/sq. sheet resistance, respectively. S. loihica PV-4 biofilms on ITO and ZnO produced maximum current density of 3.38 and 0.25 µA/cm2, respectively. Our results suggest that ZnO has potential for the growth and characterization of EABs.