Background As the field of off-road cycling is usually remote areas with limited access to medical care, off-road cyclists are at higher risk of animal attacks and related injuries. Case presentation We report two cases of dog attacks in off-road cycling and discuss the basic principles in prevention and management of such incidents. The cyclists received all 5-dose regimen of the rabies vaccine and returned to sport after 6 weeks. During 6-month follow-up period, no complications were observed. Conclusions To reduce the probability of dog attacks and its complications, the off-road cyclists should be familiar with some basic principles. They should also be educated about initial on-site management of the related injuries, which may have a great impact on decreasing further complications.