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Amiloride-sensitive channels in marginal cells in the stria vascularis of the guinea pig cochlea

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
172
Identifiers
DOI: 10.1016/0304-3940(94)90687-4
Keywords
  • Amiloride
  • Stria Vascularis
  • Sodium Channel
  • Endolymph

Abstract

Abstract We examined marginal cells in stria vascularis for the presence of amiloride-sensitive Na + channels, a possible pathway for maintaining a low Na + concentration in the endolymph. Whole-cell voltage-clamp experiment shows that amiloride at 1 μM concentration reversibly reduces inward current more than outward current. Immunogold-labeling method shows that the luminal and lateral membrane have antigenic sites for these antibodies. These observations indicate the presence of amiloride-sensitive channels in the marginal cell. If amiloride-sensitive channels in the luminal membrane are highly selective to Na +, they could be an efficient pathway for Na + uptake from the endolymph. In the basolateral membrane, amiloride-sensitive Na + channels may make a relatively small contribution to the unusual resting potential.

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