Abstract The effect of ozone treatment on total phenol, flavonoid, and vitamin C content of fresh-cut honey pineapple, banana ‘pisang mas’, and guava was investigated. The fresh-cut fruits were exposed to ozone at a flow rate of 8 ± 0.2 ml/s for 0, 10, 20, and 30 min. The antioxidant capacity of the fruits was evaluated by measuring the ferric reducing/antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging activity. Total phenol and flavonoid contents of pineapple and banana increased significantly when exposed to ozone for up to 20 min, with a concomitant increase in FRAP and DPPH values. The opposite was observed for guava. Ozone treatment significantly decreased the vitamin C content of all three fruits. The study shows promising results for enhancing antioxidant capacity of some fresh fruits by ozone treatment although the positive effect is compromised by a reduction in vitamin C content. Industrial relevance The preservation of antioxidant properties, storage stability and safety is of utmost importance in the minimally processed fresh food industry. Ozone has been looked into as an alternative sanitizing technology in the fresh produce industry. The results of this study indicate that ozone can be used in the minimally processed fresh food industry to enhance the antioxidant status of fresh-cut fruits.