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Comprehensive assessment of myocardial mechanics in mice using 3D cine DENSE

Journal of Cardiovascular Magnetic Resonance
Springer (Biomed Central Ltd.)
Publication Date
DOI: 10.1186/1532-429x-13-s1-p30
  • Poster Presentation
  • Medicine
  • Physics


Comprehensive assessment of myocardial mechanics in mice using 3D cine DENSE POSTER PRESENTATION Open Access Comprehensive assessment of myocardial mechanics in mice using 3D cine DENSE Lauren B Gibberman1, Xiaodong Zhong2*, Andrew D Gilliam1, Craig H Meyer1, Brent A French1, Frederick H Epstein1 From 2011 SCMR/Euro CMR Joint Scientific Sessions Nice, France. 3-6 February 2011 Introduction MRI of myocardial mechanics in mice enables the inves- tigation of the roles of individual genes and experimen- tal therapies in cardiac function. While two-dimensional tagging, HARP, and DENSE have previously been demon- strated for the mouse heart, myocardial mechanics are more comprehensively assessed using three-dimensional (3D) methods. Purpose We sought to develop 3D cine DENSE acquisition and analysis methods and to evaluate them for measuring 3D mechanics in normal mice. Methods A multiphasic volumetric spiral cine DENSE sequence with 3D displacement encoding was implemented on a small-bore 7T MRI system (Clinscan, Bruker). Magni- tude and phase images were reconstructed online, and semiautomatic segmentation methods followed by auto- matic strain, twist, and torsion calculations were imple- mented offline. Seven healthy C57Bl/6 mice were studied. For imaging, mice were anesthetized with 1.25% isoflurane, positioned in a 32 mm diameter birdcage RF coil, and maintained at 36°C. The ECG was monitored and used to trigger data acquisition. After localizer ima- ging, 3D cine-DENSE imaging was applied. DENSE sequence parameters included voxel size = 0.25 x 0.25 x 0.4 mm3, number of partitions = 21, TR = 7 ms, and number of cardiac phases = 14. The entire left ventricle (LV) was covered in a scan time of approximately 23 minutes. Results Volumetric cine DENSE data covering the entire LV of all 7 mice were analyzed. Semiautomatic segmentation took approximately 1 hour per mouse. Three-dimen- sional mechanics were quantified throughout the LV, 2Siemens Healthcare, Atlanta, GA, USA Full

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