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Green fluorescent protein-taggedEdwardsiella tardareveals portal of entry in fish

Authors
Journal
FEMS Microbiology Letters
0378-1097
Publisher
Oxford University Press
Publication Date
Volume
194
Issue
2
Identifiers
DOI: 10.1016/s0378-1097(00)00498-5
Keywords
  • Green Fluorescent Protein
  • Infection Kinetics
  • Portal Of Entry
  • Edwardsiella Tarda
Disciplines
  • Biology
  • Medicine

Abstract

Abstract The application of green fluorescent protein (GFP) to identify the portal(s) of entry of bacterial pathogens in animal hosts was studied using the fish pathogen Edwardsiella tarda and blue gourami, Trichogaster trichopterus. An immersion challenge model was utilized to mimic natural infection conditions in fish. Gastrointestinal tract, gills and the body surface of fish were found to be the sites of entry of virulent E. tarda (PPD130/91) by histological and infection kinetics studies. On the other hand, avirulent E. tarda (PPD125/87) was mainly found in the gastrointestinal tract, and the bacterial population in tissue declined over a period of 7 days.

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