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Comparison of the α and β isomeric forms of the detergent n-dodecyl-D-maltoside for solubilizing photosynthetic complexes from pea thylakoid membranes

Authors
Publisher
Uli Brandt, Fabrice Rappaport
Publication Date
Disciplines
  • Biology

Abstract

Author’s version Published in Biochimica et Biophysica Acta-Bioenergetics 1817(8), pp 1506/1515 DOI: 10.1016/j.bbabio.2011.11.001 DOI: http://dx.doi.org/10.1016/j.bbabio.2011.11.001 Title Comparison of the α and β isomeric forms of the detergent n-dodecyl-D-maltoside for solubilizing photosynthetic complexes from pea thylakoid membranes. Authors names and affiliation Cristina Paglianoa,*, Simone Bareraa,Fabiana Chimirria,Guido Saraccoa, James Barbera,b,* aDepartment of Materials Science and Chemical Engineering - BioSolar Lab, Politecnico di Torino, Viale T. Michel 5, 15121 Alessandria, Italy bDivision of Molecular Biosciences, Department of Life Sciences,Imperial College London, London SW7 2AZ, United Kingdom Corresponding authors *Corresponding authors. E-mail: [email protected]; Tel. No. +39 131 229301; Fax No. +39 131 229344;and E-mail: [email protected]; Tel. No. +44 207 594 5266; Fax. No. +44 207 594 5267 1 Abstract Mild non-ionic detergents are indispensable in the isolation of intact integral membrane proteins and protein- complexes from biological membranes. Dodecylmaltoside (DM) belongs to this class of detergents being a glucoside-based surfactant with a bulky hydrophilic headgroup composed of two sugar rings and a non- charged alkyl glycoside chain. Two isomers of this molecule exist, differing only in the configuration of the alkyl chain around the anomeric centre of the carbohydrate head group, axial in α-DM and equatorial in β- DM. In this paper, we have investigated the solubilizing properties of α-DM and β-DM on the isolation of photosynthetic complexes from pea thylakoids membranes maintaining their native architecture of stacked grana and stroma lamellae. Exposure of these stacked thylakoids to a single step treatment with increasing concentrations (5-100 mM) of α-DM or β-DM resulted in a quick partial or complete solubilization of the membranes. Regardless of the isome

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