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A method for identifying Ca ionophores using a unicellular organism

Authors
Journal
Journal of Pharmacological Methods
0160-5402
Publisher
Elsevier
Publication Date
Volume
3
Issue
3
Identifiers
DOI: 10.1016/0160-5402(80)90005-4
Keywords
  • Ca Ionophores
  • Intracellular Ca
  • Divalent Ionophores

Abstract

Abstract A method is described for identifying Ca ++ ionophores using the protozoa Actinosphaerium. This organism is a free-floating spherical form with filamentous axopods radiating from the surface. The axopods are composed of microtubules that disaggregate upon exposure to intracellular Ca ++. In the presence of an appropriate ionoprfore, extracellular Ca ++ will pass the cell membrane, enter the cell, and disrupt microtubules. The resultant shortening of axopods can be easily quantified under a microscope. This system may prove to be a valuable research tool in the identification of compounds that possess the ability to facilitate transmembrane Ca ++ movement.

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