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Improvement of isoflavone aglycones production using β-glucosidase secretory produced in recombinantAspergillus oryzae

Authors
Journal
Journal of Molecular Catalysis B Enzymatic
1381-1177
Publisher
Elsevier
Publication Date
Volume
59
Issue
4
Identifiers
DOI: 10.1016/j.molcatb.2008.11.013
Keywords
  • β-Glucosidase
  • Isoflavone
  • Aglycone
  • Aspergillus Oryzae
  • Superoxide Dismutase (Sodm) Promoter

Abstract

Abstract β-Glucosidase (BGL1) from Aspergillus oryzae was efficiently produced in recombinant A. oryzae using sodM promoter-mediated expression system. The yield of BGL1 was 960 mg/l in liquid culture, which is 20-fold higher than the yield of BGL1 produced using the yeast Saccharomyces cerevisiae. Recombinant BGL1 converted isoflavone glycosides into isoflavone aglycones more efficiently than β-glucosidase from almond. In addition, BGL1 produced isoflavone aglycones even in the presence of the insoluble form of isoflavone glycosides.

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