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Magnesium-dependent non-specific binding of [125I] prolactin to myelinated tracts in the rat central nervous system

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
158
Issue
3
Identifiers
DOI: 10.1016/0006-291x(89)92819-2

Abstract

Abstract An in vitro autoradiographic assay was used in identifying a magnesium-dependent, non-specific binding of [ 125I] prolactin to myelinated fiber tracts in the rat brain. Frozen tissue sections were incubated for 18 h at 4°C in media which included [ 125I] prolactin alone or with a 500 fold excess of unlabelled prolactin. Magnesium in the incubation medium caused a non-specific binding of radiolabelled prolactin to the myelinated fiber tracts in the brain. In contrast, calcium did not facilitate prolactin non-specific binding to myelin. Hence, calcium should optimize the detection of specific prolactin binding sites in the brain by in vitro autoradiographic or radioreceptor assays.

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