Affordable Access

Publisher Website

Arylsulphatases A and B in human diploid fibroblasts: Differential assay with 4-methylumbelliferylsulphate and AgNO3

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
93
Issue
1
Identifiers
DOI: 10.1016/0009-8981(79)90247-x
Disciplines
  • Medicine

Abstract

Abstract A new technique is introduced for the differential assay of arylsulphatases A and B in centrifuged homogenates of cultured human skin fibroblasts, using 4-methylumbelliferyl-sulphate as a substrate and AgNO 3 as a selective inhibitor of arylsulphatase A. The method can be applied in the diagnosis of metachromatic leucodystrophy, mucopolysaccharidosis type VI and mucosulphatidosis. Normal arylsulphatase activities were found in fibroblasts derived from patients with mucopolysaccharidoses types II, III-A and IV, known to be caused by deficiencies of various other sulphatases.

There are no comments yet on this publication. Be the first to share your thoughts.