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Temperature Effects on Pea Plants Probed by Simultaneous Measurements of the Kinetics of Prompt Fluorescence, Delayed Fluorescence and Modulated 820 nm Reflection

Authors
Journal
PLoS ONE
1932-6203
Publisher
Public Library of Science
Publication Date
Volume
8
Issue
3
Identifiers
DOI: 10.1371/journal.pone.0059433
Keywords
  • Research Article
  • Biology
  • Biochemistry
  • Plant Biochemistry
  • Photosynthesis
  • Bioenergetics
  • Molecular Cell Biology
  • Plant Cell Biology
  • Chloroplast
  • Cellular Stress Responses
  • Plant Science
  • Plant Ecology
  • Plant-Environment Interactions
  • Plant Physiology
Disciplines
  • Ecology
  • Geography

Abstract

Simultaneous in vivo measurements of prompt fluorescence (PF), delayed fluorescence (DF) and 820-nm reflection (MR) were made to probe response of pea leaves to 40 s incubation at high temperatures (25–50°C). We interpret our observation to suggest that heat treatment provokes an inhibition of electron donation by the oxygen evolving complex. DF, in a time range from several microseconds to milliseconds, has been thought to reflect recombination, in the dark, between the reduced primary electron acceptor QA– and the oxidized donor (P680+) of photosystem II (PSII). The lower electron transport rate through PSII after 45 and 50°C incubation also changed DF induction. We observed a decrease in the amplitude of the DF curve and a change in its shape and in its decay. Acceleration of P700+ and PC+ re-reduction was induced by 45°C treatment but after 50°C its reduction was slower, indicating inhibition of photosystem I. We suggest that simultaneous PF, MR and DF might provide useful information on assessing the degree of plant tolerance to different environmental stresses.

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