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Desorption of adherent bacteria from a solid hydrophobic surface by oil

Authors
Journal
Journal of Microbiological Methods
0167-7012
Publisher
Elsevier
Publication Date
Volume
1
Issue
4
Identifiers
DOI: 10.1016/0167-7012(83)90028-3
Keywords
  • Acinetobactor
  • Adherence
  • Desorption
  • Hydrophobicity
  • Oils
  • Polystyrene

Abstract

Abstract Oils efficiently desorbed bacteria from a solid surface (polystyrene). Desorption was measured qualitatively by staining techniques, and quantitatively by following the outgrowth kinetics of cells remaining on the plastic surface following the washing procedure. Approximately 97% of Acinetobacter calcoaceticus RAG-1 cells adhering to a polystyrene cuvette were removed following two successive washings in the presence of olive oil.s Results suggest that oils may serve as potent, non-toxic desorbing agents to remove attached hydrophobic bacteria from surfaces.

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