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Ecdysone-binding proteins in haemolymph and tissues ofDrosophila hydeilarvae

Authors
Journal
Journal of Insect Physiology
0022-1910
Publisher
Elsevier
Publication Date
Volume
20
Issue
11
Identifiers
DOI: 10.1016/0022-1910(74)90044-4
Disciplines
  • Biology

Abstract

Abstract An α-ecdysone-binding protein fraction, approx. mol. wt. 120,000, has been demonstrated in haemolymph of Drosophila hydei late third instar larvae. The protein has been partly characterized by Sephadex G-25 filtration, hydroxylapatite chromatography, density gradient centrifugation, and ezyme digestion experiments. The protein-steroid complex appears to be heat stable. Binding of labelled ecdysone to the protein fraction is significantly reduced in competition experiments using unlabelled ecdysones. An ecdysone-binding protein fraction has been detected in hand-isolated total alimentary tract tissues (predominantly midgut, Malpighian tubules, and salivary glands) and in mass-isolated midgut and Malpighian tubules. The sedimentation properties of this protein-hormone complex are similar to those of the complex found in haemolymph.

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