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Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens

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  • It Has Been Proposed That Geobacter Sulfurreducens Requires Conductive Pili For Long-Range Electron
  • In Order To Investigate This Further
  • We Constructed A Strain Of G
  • Sulfurreducens
  • Designated Aro-5
  • Which Produced Pili With Diminished Conductivity
  • This Was Accomplished By Modifying The Amino Acid Sequence Of Pila
  • The Structural Pilin Protein
  • An Alanine Was Substituted For Each Of The Five Aromatic Amino Acids In The Carboxyl Terminus Of Pil
  • The Region In Which G
  • Sulfurreducens Pila Differs Most Significantly From The Pilas Of Microorganisms Incapable Of Long-Ra
  • Strain Aro-5 Produced Pili That Were Properly Decorated With The Multiheme C-Type Cytochrome Omcs
  • Which Is Essential For Fe(Iii) Oxide Reduction
  • However
  • Pili Preparations Of The Aro-5 Strain Had Greatly Diminished Conductivity And Aro-5 Cultures Were Se
  • Current Production Of The Aro-5 Strain
  • With A Graphite Anode Serving As The Electron Acceptor
  • Was Less Than 10% Of That Of The Control Strain
  • The Conductivity Of The Aro-5 Biofilms Was 10-Fold Lower Than The Control Strain'S
  • These Results Demonstrate That The Pili Of G
  • Sulfurreducens Must Be Conductive In Order For The Cells To Be Effective In Extracellular Long-Range
  • Importance Extracellular Electron Transfer By Geobacter Species Plays An Important Role In The Bioge
  • For Example
  • Microbial Reduction Of Fe(Iii) Oxide Is One Of The Most Geo-Chemically Significant Processes In Anae
  • Aquatic Sediments
  • And Aquifers
  • And Geobacter Organisms Are Often Abundant In Such Environments
  • Geobacter Sulfurreducens Produces The Highest Current Densities Of Any Known Pure Culture
  • And Close Relatives Are Often The Most Abundant Organisms Colonizing Anodes In Microbial Fuel Cells
  • The Finding That A Strain Of G
  • Sulfurreducens That Produces Pili With Low Conductivity Is Limited In These Extracellular Electron T

Abstract

Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens - DTU Orbit (16/05/14) Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens - DTU Orbit (16/05/14) Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens Vargas, M., Malvankar, N. S., Tremblay, P-L., Leang, C., Smith, J. A., Patel, P., Synoeyenbos-West, O., Nevin, K. P. & Lovley, D. R. 2013 In : mBio. 4, 2, p. Article No.: e00105-13 Publication: Research - peer-review › Journal article – Annual report year: 2013

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