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Method for quantitative determination of free and peptide-linked tryptophan after reaction with 2-hydroxy-5-nitrobenzyl bromide

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
11
Issue
3
Identifiers
DOI: 10.1016/0003-2697(65)90073-4
Disciplines
  • Biology
  • Mathematics

Abstract

Abstract A method for quantitative determination of tryptophan in the presence of 20hydroxy-5-nitrobenzyl bromide substituted tryptophan is described. The procedure does not require removal of the excess reagent and can be applied to intact proteins substituted with the reagent. The procedure involves applying mathematically derived correction factors to the analysis data obtained from the Spies and Chambers method for tryptophan determination.

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