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Isolation and characterization of pili (fimbriae) fromSynechocystisCB3

FEMS Microbiology Letters
Oxford University Press
Publication Date
  • Pili
  • Fimbriae
  • Cyanobacteria
  • Synechocystis


Abstract Synechocystis CB3, isolated from the Gulf of Finland, was covered by innumerable flexible pili (fimbriae) with an approximate diameter of 6 nm. The Synechocystis CB3 pili had a tendency to attach side by side thus forming characteristic bundles consisting of several dozens of individual pilus filaments. The Synechocystis CB3 pili were isolated and purified using deoxycholate and urea, and found to be very similar to other bacterial pili in their subunit M r (21 kDa) and amino acid composition (46% hydrophobic amino acids). The amino acid analysis revealed also small amounts of glucosamine in the pilus preparation.

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