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Evaluation of bioactive compounds produced by Nocardia levis MK-VL_113 & Streptomyces tendae TK-VL_333 for cytotoxic activity

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Medknow Publications & Media Pvt Ltd
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Keywords
  • Correspondence
Disciplines
  • Physics

Abstract

Sir, Natural products are the logical starting point for discovering new drugs as evidenced by the discovery of penicillin1. More than 60 per cent of approved antitumour drugs are derived from natural compounds23. Drugs with anticancer properties including anthracycline, bleomycin, actinomycin and mitomycin have been isolated from actinobacteria45. We have earlier reported isolation and characterization of bioactive metabolites for the first time from Nocardia levis MK-VL_113 and Streptomyces tendae TK-VL_3336–10. In this study these bioactive metabolites were tested for cytotoxicity which is the pre-requisite assay for testing anticancer activity. For the extraction and purification of bioactive metabolites produced by N. levis and S. tendae, the pure cultures of the strains grown in seed medium (Yeast extract-malt extract-dextrose broth) were transferred individually to optimized fermentation media (Sucrose-tryptone broth for N. levis6 and galactose-tyrosine broth for S. tendae7 under aseptic conditions. The fermentation process was turned off after 96 h of incubation and the culture broths were collected, extracted with ethyl acetate and evaporated to vacuum. The crude residues thus obtained were subjected to purification by using chromatographic techniques (silica gel column, thin layer and semi-preparative high performance liquid chromatography) and the structure of the pure bioactive compounds was elucidated and confirmed on the basis of Fourier transform infrared (FTIR), Electron impact mass (EIMS)/Electron spray ionization mass (ESIMS) spectrometry and nuclear magnetic resonance (1H NMR and 13C NMR) spectroscopy8–10. Screening of secondary metabolites obtained from N. levis led to the identification of bioactive compounds namely 1-phenylbut-3-ene-2-ol, bis-(2-ethylhexyl) phthalate, bis-(5-ethylheptyl) phthalate and a partially purified fraction containing phenylethyl alcohol, dibutyl phthalate and 1,2-benzenedicarboxylic acid, 3-nitro89. Five bioactive compounds namely 1H-indole-3-carboxy

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