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The type 1 copper site of pseudoazurin: Axialandrhombic

Authors
Journal
Journal of Inorganic Biochemistry
0162-0134
Publisher
Elsevier
Volume
137
Identifiers
DOI: 10.1016/j.jinorgbio.2014.03.016
Keywords
  • Blue Copper Sites
  • Pseudoazurin
  • Type 1 Copper
  • 275 Ghz Epr
  • Absorption Spectrum
  • Plastocyanin

Abstract

Abstract We report on a high-frequency electron-paramagnetic-resonance study of the type 1 copper site of pseudoazurin. The spectra fully resolve the contribution of a nearly axial spectrum besides the rhombic spectrum, which unequivocally proves the existence of two conformations of the copper site. Pseudoazurins have been considered from Achromobacter cycloclastes including eight mutants and from Alcaligenes faecalis. The two conformations are virtually the same for all pseudoazurins, but the rhombic/axial population varies largely, between 91/9 and 33/67. These observations are discussed in relation to optical absorption spectra and X-ray diffraction structures. A similar observation for fern plastocyanin from Dryopteris crassirhizoma suggests that dual conformations of type 1 copper sites are more common.

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