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Functional coupling between vanillate-O-demethylase and formaldehyde detoxification pathway

Authors
Journal
FEMS Microbiology Letters
0378-1097
Publisher
Oxford University Press
Publication Date
Volume
253
Issue
2
Identifiers
DOI: 10.1016/j.femsle.2005.09.036
Keywords
  • Protocatechuate
  • Formaldehyde
  • Vanillate-O-Demethylase
  • Formaldehyde Dehydrogenase
  • S-Formylglutathione Hydrolase
  • Bioconversion

Abstract

Abstract Pseudomonas putida vanillate- O-demethylase consisting of VanA and VanB was expressed in Escherichia coli strain K-12. Recombinant E. coli strain K-12 cells expressing VanAB efficiently converted vanillate into protocatechuate with glucose consumption. Mutant lacking either pgi or zwf showed higher or lower converting activity than the parental strain, respectively. Formaldehyde, which is the by-product of the demethylation, was converted into formate in the cellular reaction. Formate accumulation was blocked by gene disruption of the E. coli frmA that coded glutathione-dependent formaldehyde dehydrogenase.

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