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31P NMR study of the interactions between oligodeoxynucleotides containing (6-4) photoproduct and Fab fragments of monoclonal antibodies specific for (6-4) photoproduct

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
429
Issue
2
Identifiers
DOI: 10.1016/s0014-5793(98)00582-1
Keywords
  • (6-4) Photoproduct
  • 31P Nuclear Magnetic Resonance
  • Antibody
  • Fab
  • Dna Damage
  • Protein-Nucleic Acid Interaction
Disciplines
  • Chemistry

Abstract

Abstract A 31P nuclear magnetic resonance (NMR) study of the interactions between oligonucleotides containing the (6-4) photoproduct and the Fab fragments of monoclonal antibodies (64M3 and 64M5) recognizing the (6-4) photoproduct is reported. The 31P chemical shift data indicate that backbone conformation of (6-4) adduct is affected by the presence of flanking oligodeoxynucleotides, and (6-4) adducts with different backbone conformations are accommodated in the antigen binding sites of these antibodies. It was also revealed that epitopes for these antibodies consist of not only the (6-4) adduct but the flanking di- or tri-deoxynucleotides on both the 5′ and 3′ sides as well.

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