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Acid-fast staining of haplosporidian spores in relation to oyster pathology

Authors
Journal
Journal of Invertebrate Pathology
0022-2011
Publisher
Elsevier
Publication Date
Volume
7
Issue
2
Identifiers
DOI: 10.1016/0022-2011(65)90026-1

Abstract

Abstract Infected tissues from the oyster Crassostrea virginica (Gmelin), were subjected to a modified Ziehl-Neelsen carbol fuchsin technique in order to determine whether haplosporidian parasites produced spores with acid-fast properties. Minchinia costalis ( Wood and Andrews, 1962) Sprague, 1963, sporoplasm was found to be quite acid-fast, remaining bright red after the acid destaining procedure. A combined staining technique employing the Ziehl-Neelsen procedure with Harris' hematoxylin and eosin was devised and applied to oyster samples collected from Pocomoke Sound, Maryland over a 3-year period. Although 235 of these oysters were infected with a haplosporidian-like organism labeled MSX, no spores with acid-fast properties were observed.

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