Affordable Access

Publisher Website

Mathematical modelling of the washing phase of a semi-batch affinity ultrafiltration system for enzyme separation

Authors
Journal
Biochemical Engineering Journal
1369-703X
Publisher
Elsevier
Publication Date
Volume
9
Issue
1
Identifiers
DOI: 10.1016/s1369-703x(01)00120-6
Keywords
  • Affinity Ultrafiltration
  • Trypsin Purification
  • Washing Phase Modelling
Disciplines
  • Mathematics

Abstract

Abstract A semi-batch affinity ligand ultrafiltration system used for the separation of trypsin from a simulated mixture containing trypsin (MW: 23,800) and chymotrypsin (MW: 27,000) has been studied using regenerated cellulose membrane (MWCO: 30,000) and soybean trypsin inhibitor (STI) as ligand. The concentrations of both trypsin and chymotrypsin are 0.02 mg/ml in the mixture while the ratio between STI and trypsin has been maintained at 50:1. Operating pressure of the ultrafiltration module was in the range of 199.4–395.5 kPa. Concentration of trypsin in the ultrafiltration module went down from its initial value to zero within 160 s whereas chymotrypsin took 300 s to go down to zero. Two deterministic mathematical models have been developed and have been compared with the experimental observations.

There are no comments yet on this publication. Be the first to share your thoughts.